This paper presents a comparative evaluation of methods for automated voxel-based spatial mapping in diffusion tensor imaging studies. Such methods are an essential step in computational pipelines and provide anatomically comparable measurements across a population in atlas-based studies. To better understand their strengths and weaknesses, we tested a total of eight methods for voxel-based spatial mapping in two types of diffusion tensor templates. The methods were evaluated with respect to scan-rescan reliability and an application to normal aging. Looking forward, these results can potentially help to interpret results from existing white matter imaging studies, as well as provide a resource to help in planning future studies to maximize reliability and sensitivity with regard to the scientific goals at hand.